c. Challenge: You are working with a neuronal cell line isolated from a human brain tumor. It appears to have a neuronal phenotype when grown in culture on laminin coated dishes but appears to have more of a typical round, small (3 to 5 micrometer) phenotype when grown on a standard, collagen-only coated dish or a poly-lysine coated (positive charge only - no ECM component) dish. Under all 3 cases the cells attach to the substrate. You suspect that the reason the neuronal phenotype is supported by the laminin coating is due to the ability of laminin, but not collagen or poly-lysine, to bind to the integrin receptors RGD binding domain thus triggering a molecular cascade that leads to the expression of the neuronal phenotype. How might you test this hypothesis

Answers

Answer 1

Answer:

This process can be performed using immunofluorescence analysis via antibodies.

Explanation:

To test for the hypothesis

To test for the hypothesis we have to  analyze whether the neuronal phenotype is due to the binding of laminin to integrins, and this will enable us to determine which integrins are abundantly expressed on the neuronal cell membrane. This process can be performed using immunofluorescence analysis via antibodies

Finally when no differentiation markers are expressed in the laminin coated plates with mutant cells we can now say that it expresses  integrins


Related Questions

7. Photosynthesis is impossible at a depth of 1000 m because: A it is too hot B only blue light penetrates this far C no light penetrates this far D it is too cold 8. Which of the following is not true about the viscosity of water? A if it were more viscous the heart would not be able to move the blood through the blood vessels B if it were less viscous the organelles would not be suspended in the cytoplasm C if it were less viscous the heart would not be able to move blood through the blood vessels D if it were more viscous the organelles would be damaged in the cytoplasm 9. The high surface tension of water is due to: A unbalanced hydrogen bonding in the body of the water B balanced hydrogen bonding in the body of the water C balanced hydrogen bonding at the surface of the water D unbalanced hydrogen bonding at the surface of the water 10. One benefit of ice being less dense than water is: A water freezes from the top down and water is insulated under the ice B water freezes from the top down and the water under the ice is cooled more quickly C water freezes from the bottom up and the remaining water cools more slowly D water freezes from the bottom up and the remaining water cools more quickly​

Answers

Answer:

bh

Explanation:

Answer: The answer for 7 is C no light penetrates that far

8  d

9 D

10 A

Explanation:

minerals___.
- can’t be compounds
- are the same as rocks
- can be gases
- must be inorganic

Answers

Answer:

minerals must be inorganic

which two statements best describes the position of a coastal area during a high tide?

a. it is parallel to earths axis
b. it faces directly toward or away from the moon
c. it is parallel to earths orbit plane
d. it is perpendicular to the moon

Answers

The two statements that best describes the position of a coastal area during a high tide is that it is perpendicular to the moon which is therefore denoted as option D.

What is a Tide?

This is referred to the are very long-period waves that move through the ocean in response to the forces exerted by the moon and sun an there are two types which are high and low tide.

The position of coastal ares during high tide is that it is perpendicular to the moon which is why the side of the Earth closest to the Moon experiences the Moon's pull the strongest, and this causes the seas to rise, creating high tides.

Read more about Tide here https://brainly.com/question/2604305

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Answer:

The answer would be B

Explanation:

Please help! I will give brainliest!⚠️⚠️

Answers

B, because it moves away from eachother

Can an intersex person cause a girl to get pregnant?

Answers

Explanation:

If an intersex person has a uterus, they may be able to carry a child.

what is chromosomes ​

Answers

Answer:

a chrosome is made up of proteins and DNA organized into genes each cell normally contains 23 pairs of chrosomes.

Chromosomes are structures formed by a DNA molecule associated with protein molecules. In prokaryotic cells, we observe a circular chromosome; in eukaryotes, chromosomes are linear and located inside the nucleus.

Is there an impact on the environment if blue whales went extinct?

Answers

Answer:

Yes.

Explanation:

Blue whales are the largest animals in the world, and they are the apex predator in the ocean, meaning they help to regulate the population of other species in the ecosystem. As with any species, the loss of blue whales would cause imbalances in the food chain, which would have far-reaching effects throughout the ocean and its inhabitants. In addition, blue whales help maintain the balance of carbon dioxide in the ocean which could be lost if the species were to go extinct.

Question 4 of 29
Which of the following contains chemical energy?
A. Plants
B. Light
C. Height
D. Winds

Answers

All of the above




Lol
A. Plants because of photosynthesis

Why does the water condense in the first tank when the oxygen and nitrogen do not?

Answers

the molecules in those gases have very little kinetic energy to push molecules apart. Because of this, the attraction between the molecules pulls them together and condenses the gases into a liquid, just like the water in the first tank. ... When the nitrogen evaporates, the liquid left behind is mostly oxygen.

multiple choice question

Answers

Answer:

C) Sodium ions

On the planet Kermit, in the Muppet population, there are two autosomal loci of interest. The first locus has alleles A and a, and the second locus has alleles B and b. A and B are the dominant alleles at the respective loci. The loci act in an epistatic manner, identical to the way two genes interact to produce coat color in labradors. The phenotype here is muppet hair color. We have the following results:

Muppet hair color as a function of two-locus genotypes

Two-locus genotype Muppet hair color
A_B_ Cookie monster blue
aaB_ Elmo red
__bb Janice blonde

If two doubly-heterozygous Cookie-monster blue-haired muppets mate, and produce 1000 children, which of the follow values most closely represents the expected number of Janice blonde-haired offspring?

a. 12.
b. 260.
c. 252.
d. 512.
e. 240.

Answers

Answer:

The values that most closely represent the expected number of Janice blonde-haired offspring is 252 individuals, option C.

Explanation:

Available data:

Two diallelic genes acting in a recessive epistatic manner to express hair colorAllele A is dominant over the allele aAllele B is dominant over the allele bA_B_ Cookie monster blue aaB_ Elmo red __bb Janice blonde

When talking about epistasis, we are referring to an interruption. These are interactions between genes located in different loci in the same chromosome. An epistatic gene can alter, influence, or suppress the expression of a hypostatic gene. When the epistatic gene is recessive, the interaction is known as recessive epistasis.  

Observing the altered phenotype proportions of the offspring can reveal which interaction is operating in the trait determination.  

In recessive epistasis, the proportion is 9:3:4.

Cross: Between two doubly-heterozygous

Parentals) AaBb    x    AaBb

Phenotype) Cookie-monster blue-haired individuals

Gametes)  AB, Ab, aB, ab

                 AB, Ab, aB, ab

Punnett square)     AB       Ab        aB         ab

                 AB      AABB    AABb  AaBB    AaBB

                 Ab      AABb    AAbb   AaBb    Aabb

                  aB     AaBB     AaBb   aaBB    aaBb

                  ab     AaBb      Aabb   aaBb    aabb

F1) 1000 individuals

     9/16 individuals are expected to be Cookie-monster blue-haired, A-B-

     3/16 individuals are expected to be Elmo Red, aaB-

     4/16 individuals are expected to be Janice blond, A-bb + aabb

16 ------------------------------------------- 1000 individuals

4 Janice blond individuals ---------- X = 250 individuals

So, 250 individuals are expected to be Janice blonde. The observed number might vary a little bit from this value.  The values that most closely represent the expected number of Janice blonde-haired offspring is c = 252 individuals

what is a non example of crystallization​

Answers

Answer:

Microscopically, a single crystal has atoms in a near-perfect periodic arrangement; a polycrystal is composed of many microscopic crystals (called "crystallites" or "grains"), and an amorphous solid (such as glass) has no periodic arrangement even microscopically.

Most inorganic solids are not crystals but polycrystals, i.e. many microscopic crystals fused together into a single solid. ... The third category of solids is amorphous solids, where the atoms have no periodic structure whatsoever. Examples of amorphous solids include glass, wax, and many plastics.

In condensed matter physics and materials science, an amorphous (from the Greek a, without, morphé, shape, form) or non-crystalline solid is a solid that lacks the long-range order that is characteristic of a crystal. In some older books, the term has been used synonymously with glass.

Explanation:

This chapter highlights mesocrystals as an interesting example of particle‐mediated, non‐classical crystallization processes. Mesocrystals — the shortened name for mesoscopically structured crystals — are superstructures composed of nanoparticles, being arranged three‐dimensionally in crystallographic register. Mesocrystals are often only intermediate structures in a non‐classical crystallization pathway leading to a final single crystal by nanoparticle fusion. Therefore, they are difficult to detect. Although mesocrystals were initially described for synthetic systems, recent investigations have revealed an increasing number of bio‐mineral systems which appear to be mesocrystals, but which so far have been considered to be single crystalline, including nacre and sea urchin spines. This chapter briefly defines non‐classical crystallization processes, provides some examples of synthetic mesocrystals and mesocrystals in biomineralization, and attempts to provide some insight into their formation mechanisms, despite their being as yet largely unexplored.

23. Which is NOT an electromagnetic wave?
A. sound
C. infrared wave
B. microwave
D. ultraviolet ray​

Answers

Answer:

sound

Explanation:

electromagnetic waves travel at the speed of light, for example when there is a thunder storm the lightening is seen before the thunder is heard


Carbon dioxide moves through the plasma membrane through the process of

A. osmosis
B. active transport
C. diffusion
D. facilitated diffusion
E. both diffusion and osmosis​

Answers

As oxygen-rich (and carbon dioxide-poor) blood travels by a cell the oxygen diffuses through the cell membrane to the area of lower concentration inside the cell. It can do this easily because the oxygen molecule (O2) is very small and has no charge or polarity. The oxygen is used up rapidly by mitochondria. This rapid consumption causes oxygen to constantly move into the cell from the blood. Therefore the answer is C

Can anyone answer this science question? No links please

Which constellation is found at approximately -15° declination and 4 hours 40
minutes right ascension?

Lepus

Canis Major

Eridanus

Taurus

Answers

Answer:

Eridanus.

Explanation:

Eridanus is a constellation recorded in antiquity by Ptolemy and is one of the 88 official constellations established by the International Astronomical Union.  

It is located south of the zodiac constellations Aries and Taurus, and includes about 126 stars visible to the naked eye from 1st to 6th magnitude. It is considered the longest of the constellations: it starts from the celestial equator and ends at the 60th southern celestial parallel.

If there were 1176 seeds collected, predict how many would be axial?
Predict how many seeds would be homozygous dominant?
homozygous recessive:
terminal?
heterozygous?

Answers

According to the given that actual flower position is dominant over terminal flower.

What is a homozygous dominant plant?

When an organism possesses two copies of the same dominant allele, it is said to be homozygous dominant, and when it possesses two copies of the same recessive gene, it is said to be homozygous recessive.

When an organism is homozygous, it contains two copies of the same allele for a gene. When two copies of the same dominant allele or two copies of the same recessive allele are present in an organism, it is said to be homozygous dominant or homozygous recessive.

When an organism is heterozygous, it has two distinct alleles of the same gene. For instance, pea plants can have either homozygous dominant (red-red) or heterozygous red blooms (red-white). They are homozygous recessive if they have white blooms (white-white). Heterozygous carriers are always present.

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True or False: Coal is the cheapest available source of energy.
True
False

Answers

Answer:

True

Explanation:

A coal is cheapest or least expensive among non- renewable energy.

... But it is not the cheapest among all because solar energy, wind energy etc


Explain how fish and sharks breathe. what are some differences?

Answers

uhhhhh they breath bc they’re animals
They breathe through their gills

What is added during DNA extraction to lyse cells?

Answers

Answer:

There are 3 basic steps involved in DNA extraction, that is, lysis, precipitation and purification. In lysis, the nucleus and the cell are broken open, thus releasing DNA. ... Alcohol (e.g isopropanol or ethanol) is then added, causes precipitation of DNA from the aqueous solution since it does not dissolve in alcohol.

Explanation:

What is a functioning or functional penis?

Answers

Answer:

A functioning or functional penis is when a penis is working meaning when the penis reacts to a woman naked thats a functional penis

The ability to perform or get stimulation to arousal such as a B0ner

filtered for "brainly purposes"

What is the probability of tossing a coin and getting heads three times in a row?

What is the chance that the next toss will be heads? What is the chance that the next toss will be tails?

Answers

12.5, 8.125,91.875

just do the math, 50/4 then 8 and then subtract

The solid below is made of twelve identical
rectangular prisms. The overall dimensions of the composite solid are 36 inches (in.) by 36 inches by 24 inches. What is the volume, in cubic inches, of a single prism?

Whoever answers correctly will get Brainliest.

Answers

Answer:

Hewo! Mizuki here to help! The correct answer would be option A!

Explanation:

Oki! So.... we find out the length, width, and height first!

The equation for finding the length: 36 ÷ 2

And the answer will be 18, make sure to memo it if your following along!

The equation for finding width: 36 ÷ 3

And the answer will be 12, so yeah(I tried it with 2 because it was 2 prism long, but it turned out to be a number not on the list [3888])

The equation for finding height: 24 ÷ 2

And the answer will be 12!

Soo... we multiply the length, width, and height!

18 x 12 x 12 = 2592

Since 2592 in on the list, that will be your answer!

How are clouds formed?
Explain your answer.''

Answers

Answer:

Water vapor

Explanation:

Clouds are formed when water evaporates into water vapor.

Answer:clouds mainly consist of water vapor clouds mainly consist of water vapor

A CBC specimen was rejected for testing by the hematology department. The tube was filled completely and labeled properly. What might have been the reason for rejection?

Answers

Answer:

Explanation:

The only thing I can think of is that if you are testing blood for a CBC, you are sometimes required to reject the 1st sample. Perhaps the technician forgot to take a sample that is just meant to be pitched or it was improperly labeled. I get my blood checked every other week, and the people who take it out are scrupulously careful in making sure that the first tube is labelled as waste. No one has every made the mistake of not doing it, but I can see it happening.

The question says it was properly labeled. I didn't see that. The only other thing I can think of is that the machine improperly recorded what the blood cells were and the numbers were all out of kilter.

State what is lost at each trophic level of a food chain and describe what this is used for.

Answers

Answer:

energy is lost at each trophic level and the energy is used for warmth and digestion

Explanation:

when we eat we get energy and so do animals. like an ant will eat grass and gain energy then a anteater would eat the ants and gain energy to use for digestion then a bigger animal eats the ant eater and gains its energy and so on but everytime something is eaten energy is lost

Which system of astronomical coordinates does not have its point of origin at the
Vernal Equinox?

Equatorial
Ecliptic
Celestial
Galactic

Answers

Answer:

Celestial ecliptic

Explanation:

In space seience , the ecliptic is really the conformance that represents the Sun's visible movement from around stars and planets over the span of a year; from some other perspective, it is the reflection on the celestial equator of Earth's orbit around the Sun. The constellation stars and planets are distributed around the ecliptic.

Answer:

Galactic

Explanation:

The origin of the galactic coordinate system points towards the center of the galaxy rather than towards the vernal equinox. The celestial and ecliptic coordinate systems have their point of origin towards the Vernal Equinox. The equatorial coordinate system doesn't exist.


Help me please omggg I need help I am begging y’all

Answers

Answer:

the answer is D

Explanation:

it is better explain and it could pullout water

The main types of molecules in your body are water and carbon-containing organic molecules. To continue to live, you need to consume organic molecules in your food. What does your body use these organic molecules for?

Answers

Answer:

for production of energy

Explanation:

to enable the action of heart beat, keeping the body warm, to build body tissues, etc, while facilitates ones living

Name the characteristic of living organism illustrated by each if activities described below.


Dressing heavily.
Bursting of the sporangium in Rhizopus.
Bending of the plant shoot towards light.
A monkey putting a yellow banana in it's mouth​

Answers

Answer:

This question relates to the qualities that help us differentiate between living and non-living things. In this question, some activities have been described. As indicated below, such activities regardless of how it is exhibited must fall under a certain trait for which living organisms are known. Hence the answer to the question is as follows:

A: Dressing heavily can be characterized as ADAPTATION

B: Bursting of the Sporangium in Rhizopus is a process that is critical to its REPRODUCTION

C: Bending of the plant shoot towards light speaks to MOVEMENT. For them, light is critical to growth so they must move towards it and away from the shade.

D:  A monkey putting a yellow banana in its mouth is called NUTRITION. Every living organism must absorb nutrients in order to remain alive and grow.

Explanation:

There are other characteristics that help to differentiate living from non-living organisms. They are best memoriZed using the following acronym:  

MR. NIGER A.D.

M: Movement

R: Respiration

N: Nutrition

I:  Irritability

G: Growth

E: Excretion

R: Reproduction

A: Adaptation

D: Death

There are other qualities such as  

possession of Cells and DNA  (a living thing may be unicellular or multicellular)ability to Metabolize, that is, ingest and convert and absorb nutrients Homeostasis: This is the ability of an organism to regulate its internal environment Interaction with living and non-living things

All the above make it easy to distinguish between matter that has life and that which doesn't.

Cheers

 

   

Classify each trait as favorable or non-favorable for different organisms
living in a jungle
Favorable trait
Non favorable trait

A thick layer of fot to help
keep the organism warmin
the cold

Green fouthers to help
Camouflage

Long strong limbs to help
climb trees

Answers

Answer:

A thick layer of fat to help keep the organism warm in the cold - non-favourable trait because jungles aren't usually very cold

Green feathers to help camouflage - favourable trait because the environment is mostly green

Long, strong limbs to help climb trees - favourable trait because jungles have many trees

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