I need 100 ml of a 20 uM solution of a protein with a molecular weight of 50,000. How much protein do I need

Answers

Answer 1

I need 0.1g of protein

HOW TO CALCULATE NUMBER OF MOLES?

The number of moles of a substance in a solution can be calculated by multiplying the volume by the molarity of the solution i.e.

no of moles (n) = Molarity (M) × Volume (V)

In this question, 100 ml of a 20 uM solution of a protein was given.

Since 1uM = 10^-6M

20uM = 20 × 10-⁶

= 2 × 10-⁵M

1L = 1000ml

100mL = 100/1000

= 0.1L

no of moles = 2 × 10-⁵M × 0.1L

no of moles = 2 × 10-⁶mol

HOW TO CALCULATE MASS:

Mass of a substance can be calculated by multiplying the molar mass and the number of moles i.e.

Mass = moles × Molar mass

Molecular mass of protein = 50,000g/mol

Mass = 2 × 10-⁶mol × 50000g/mol

Mass = 0.1g

Therefore, 0.1gram of protein is needed

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Answer 2
You will need 0.1 g of the protein.

Molarity of a solution is simply defined as the mole of solute per unit litre of water.

Mathematically, it is expressed as:

[tex]Molaity = \frac{Mole }{Volume }[/tex]

With the above information in mind, we can determine the amount of the protein needed as follow:

Step 1:

Data obtained from the question

Volume = 100 mL

Molarity = 20 μM = 20×10¯⁶ M

Molecular weight of protein = 50000 g/mol

Mass of protein=?

Step 2

Conversion of 100 mL to litre (L).

1000 mL = 1 L

Therefore,

[tex]100 mL = \frac{100 mL * 1 L}{100 mL }\\\\100 mL = 0.1 L[/tex]

Step 3

Determination of the mole of the protein

Volume = 0.1 L

Molarity = 20×10¯⁶ M

Mole of protein =?

Mole = Molarity × Volume

Mole of protein = 20×10¯⁶ × 0.1

Mole of protein = 2×10¯⁶ mole

Step 4

Determination of the mass of the protein

Mole of protein = 2×10¯⁶ mole

Molecular weight of protein = 50000 g/mol

Mass of protein =?

Mass = mole × molar mass

Mass of protein =  2×10¯⁶ × 50000

Mass of protein = 0.1 g

Therefore, the amount of the protein needed is 0.1 g

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Related Questions

The volume of real gas will change if “P” increases upto 500 atm and temperature decrease upto 0°C A. Volume increases by 0.05% B. Volume decreases by 0.05% C. Volume increases 20% D. Vol decreases 20%​

Answers

B.volume decreases by 0.05%

We have that The volume of real gas will change if “P” increases upto 500 atm and temperature decrease upto 0°C there will be

. Volume decreases by 0.05%

OPtion B

From the question we are told that

if “P” increases upto 500 atm

temperature decrease upto 0°C

Generally the equation for the ideal gas equation  is mathematically given as

P=\frac{nRT}{V}

Where

n=1

R=0.08

Therefore

The volume is directly proportional to temperature

Therefore

. Volume decreases by 0.05%

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Which of these outcomes is conclusive evidence that mixing sugar in water is a physical change?

Answers

Answer:

» Water changes from colorless to a pale colored solution.

» The solubility of water reduces because a saturated solution is formed.

» The freezing point of water decreases. [colligative property]

» The boiling point of water elevates [colligative property]

» The vapour pressure of water lowers [colligative property]

Explanation:

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Answers

Answer:

Option A is your answer

Explanation:

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Calculate the liters of a 4.40 M KCl solution to obtain 0.200 mole of KCI

Answers

Answer:

[tex]\boxed {\boxed {\sf 0.0455 \ L}}[/tex]

Explanation:

We are asked to calculate the liters given the molarity and moles in a solution.

Molarity is a measure of concentration in moles per liter. It is calculated with the following solution:

[tex]molarity= \frac{moles \ of \ solute}{liters \ of \ solution}[/tex]

The molarity of the solution is 4.40 molar of potassium chloride (KCl). 1 molar is equal to 1 mole per liter, so the molarity is also 4.40 moles of potassium chloride per liter. There are 0.200 moles of potassium chloride or solute. The liters of solution is unknown, so we can use the variable x.

molarity= 4.40 moles KCl / L moles of solute = 0.200 mol KCl liters of solution = x

[tex]4.40 \ mol \ KCl/ L = \frac{ 0.200 \ mol \ KCl}{x}[/tex]

Since we are solving for x, we must isolate the variable. First, cross multiply. Multiply the first numerator by the second denominator, then the first denominator and the second numerator.

[tex]\frac {4.40 \ mol \ KCl/ L}{1} = \frac{ 0.200 \ mol \ KCl}{x}[/tex]

[tex]4.40 \ mol \ KCl / L * x= 1 * 0.200 \ mol \ KCl[/tex]

[tex]4.40 \ mol \ KCl / L * x= 0.200 \ mol \ KCl[/tex]

x is being multiplied by 4.40 moles of potassium chloride per liter. The inverse operation of multiplication is division. Divide both sides by 4.40 mol KCl/L

[tex]\frac {4.40 \ mol \ KCl / L * x}{4.40 \ mol \ KCl / L}= \frac {0.200 \ mol \ KCl}{{4.40 \ mol \ KCl / L}}[/tex]

[tex]x= \frac {0.200 \ mol \ KCl}{{4.40 \ mol \ KCl / L}}[/tex]

The units of moles of potassium chloride cancel.

[tex]x= \frac {0.200}{{4.40 L}}[/tex]

[tex]x= 0.0454545454545 \ L[/tex]

The original measurements of liters and moles have 3 significant figures, so our answer must have the same. For the number we calculated, that is the ten-thousandth place (0.0454545454545). The 5 to the right of this place (0.0454545454545) tells us to round the 4 up to a 5.

[tex]x \approx 0.455 \ L[/tex]

There are approximately 0.455 liters in a 4.40 molar solution with 0.200 moles of solute.

If the density of HCl is 1.49 g/mL, What is the volume of 3.5 g of hydrochloric acid?


PLEASE HELP I NEED THE ANSWER ASAP!!

Answers

Answer- 2.3

Explanition-

The density of hydrochloric acid is

1.49

g/mL

, and the mass is

3.5

g

. We need to use this information to find the volume of the acid. Since the above equation shows us the relationship between density, mass, and volume, we can rearrange the equation to find the volume:

Volume

=

Mass (g)

Density (g/mL)

=

3.5

g

1.49

g/mL

=

2.349

mL

The question asks for the answer to have the correct number of significant figures. Since solving this question requires division, the measurement with the least number of significant figures decides how many significant figures the final answer should have.

1.49

has three significant figures, while

3.5

has two significant figures. Therefore, the final answer for volume can have only two significant figures, which gives the final result as:

2.3

.

The density of compound is its mass divided by volume. The volume of 3.5 g hydrochloric acid with a density 1.49 g/ml is 2.34 milliliter.

What is density ?

Density of a substance is the measure of its mass per volume. Thus how much denser is it in a particular volume is represented by the physical quantity density.

Density of a compound is dependant on its mass, volume, temperature, bond type and pressure. The ratio of density of any object to the density of water is called specific gravity.

It is given that the mass of hydrochloric acid is 3.5 g and density is 1.49 g/ml. The volume of HCl solution can be calculated as follows:

Density = mass/volume

volume = mass/density

            = 3.5 g/1.49 g/ml

            = 2.34 ml.

Hence, the volume of HCl solution is 2.34 ml.

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Is Calcium, Ca a metal or a non-metal? Explain your thinking.

Answers

Calcium is a metal. It is an alkaline earth metal. Calcium is a metal because it is in group 2 which makes it an alkaline earth metal and a powerful reducing agent that loves to react with nonmetals especially powerful oxidizing agents like fluorine and chlorine.

Answer:

Calcium is a metal, particularly,an alkali earth metal

Explanation:

It donates two electrons to form cation

Ca+2e––>Ca^2+

What is the name of this molecule?
A. Hexane
B. Hexyne
C. 2-pentane
D. Pentane

Answers

A.hexane is the name of the molecule

a) Briefly discuss hydrolysis of sicl4​

Answers

Answer:

Types of Hydrolysis

There are several types of hydrolysis, and we will look at them in brief below.

Salts: This is the most common type of hydrolysis. Hydrolysis of salts generally refers to the reaction of salt with water where it involves the interaction between cations or anions of salts and water. During hydrolysis, a salt breaks down to form ions, completely or partially depending upon the solubility factor.

Acid and Base: Acid–base-catalysed hydrolysis can be found during the hydrolysis of esters or amides. Here, the process of hydrolysis occurs when water or hydroxyl ion reacts with the carbon of the carbonyl group of the ester or amide where new compounds are formed. The products of both hydrolysis are compounds with carboxylic acid groups.

ATP: Most biochemical reactions that occur in living organisms are in the form of ATP hydrolysis which takes place with the help of enzymes acting as catalysts. The catalytic action of enzymes allows the hydrolysis or breaking down of proteins, lipids, oils, fats and carbohydrates.

Explanation:

The rate of reaction for the decomposition of hydrogen peroxide to yield water and oxygen is given by the equation: Rate = k[H202). Which of the following is indicated by this equation?

a. The rate of the reaction is unaffected by the concentration of H2O2.
b. The rate of the reaction will increase with increasing concentration of H2O2.
c. The rate of the reaction will decrease with increasing concentration of H2O2.
d. None of these

Answers

c. the rate of reaction will decrease with increasing concentration of H2O2 is false

If a box car racer is traveling at 23.6 miles per hour on a downhill track, how many seconds will it take for the racer to travel 20 meters

Answers

It will take the racer 1.9 s.

Speed is defined as the distance travelled per unit time. Mathematically, it is expressed as:

[tex]Speed = \frac{Distance }{Time}[/tex]

To solve the question given above, we'll begin by converting 23.6 miles per hour to metre per second (m/s). This can be obtained as follow:

1 mph = 0.447 m/s

Therefore,

23.6 mph = 23.6 × 0.447

23.6 mph = 10.5492 m/s

Thus, 23.6 mph is equivalent to 10.5492 m/s

Finally, we shall determine the time taken for the racer to travel 20 m. This can be obtained as follow:

Speed = 10.5492 m/s

Distance = 20 m

Time =?

[tex]Speed = \frac{Distance }{Time } \\\\10.5492 = \frac{20}{Time}[/tex]

Cross multiply

10.5492 × time = 20

Divide both side by 10.5492

[tex]Time = \frac{20}{10.5492 } \\[/tex]

Time = 1.9 s

Therefore, it will take 1.9 s for the racer to travel a distance of 20 m.

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The mass of an atom of element x is equivalent to the total mass of 7 hydrogen atoms

Answers

hydrogen mass is 1, the atom with mass number 7 is Lithium

why hydrogen has dual nature?​

Answers

Answer:

Since hydrogen has a single electron in its valence shell, it exhibits dual nature. of the periodic table, or it can obtain one electron to complete its valency of two, such as halogens from Group 17. Since it only has one electron in its outer shell, hydrogen has a dual existence.

Explanation:

This means that, like a normal halogen, a hydrogen atom can easily gain an electron to form a complete outer shell, or lose an electron to form an alkali metal. Since it already has two electrons in its outer shell, hydrogen can be diatomic, rendering it unreactive.

Find the final pressure of a gas at 150. K, if the pressure of the gas is 210. kPa at 120. K.

Answers

Answer:

final pressure = 262.5kpa

Explanation:

using Gay-Lussac's Equation

P1/T1 = P2/T2

P1 = 210kpa = 210000pa

T1 = 120k

P2 = ?

T2 = 150k

210000/120 = P2/150

1750 = P2/150

P2 = 1750 X 150

P2 = 262500pa

P2 = 262.5kpa

Hello I need help please

Answers

Answer:

A is the answer hope this helps if wrong I ont know

How does convection affect the earths atmosphere

Answers

Answer:

I think the layers of the atmosphere does temperature increase with increasing height. It is affected by convection because it heats the lower atmosphere. It is affected by conduction because the is the transfer of thermal energy. I guess

Hope this help!

Which of the following alcohols will give a positive chromic acid test?
(A) tert-butanol (B) cyclohexanol (C) pentan-3-ol
(D) both A and B (E) both B and C

Answers

the answer is both B and C

positive chromic acid test is indicated by disappearance of orange colour from chromic ions and appearance of blue-green color from Chromium (iii) ion (reduction of chromium ion from CrO4 - to Cr3+)

positive chromic test indicated functional groups that can be oxidized.

cyclohexanol can be oxidized to become cyclohexanone

and pentan-3-ol can be oxidized to become pentan-3-one

hence both B and C will show positive chromic acid test

A) tert butanol although contains alcohol functional group, cannot be further oxidized as it is a tertiary alcohol

(E) Both B and C

What is chromic acid test?

The chromic acid test uses the Jones reactant to oxidize aldehydes and alcohols and reduce the chromic acid, resulting in a color change.Positive chromic acid test is indicated by disappearance of orange color from chromic ions and appearance of blue-green color from Chromium (III) ion (reduction of chromium ion from [tex]CrO_4^- \text{to } Cr^{3+}[/tex])

Positive chromic test indicated functional groups that can be oxidized.

⇒Cyclohexanol can be oxidized to become cyclohexanone.

⇒Pentan-3-ol can be oxidized to become pentan-3-one.

Hence, both B and C will show positive chromic acid test.

A) tert-butanol although contains alcohol functional group, cannot be further oxidized as it is a tertiary alcohol.

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b. Use the balanced equation to answer the following questions.
CuSO4(aq) + 2NaOH(aq) —->Cu(OH)2(s) + Na2SO4(aq)

i. What is the ratio of moles of CuSO4 to moles of NaOH?
ii. If 638.44 g CuSO4 reacts with 240.0 NaOH, which is the limiting reagent?
iii. Using the limiting reagent to determine how many grams of Cu(OH)2 should precipitate out in the reaction.
iv. If only 174.6g of Cu(OH)2 precipitate were actually collected from the reaction, what would the percent yield be?

Answers

Answer:

i. 1 : 2

ii. NaOH is the limiting reagent

iii. 292.5g

iv. 59.69%

Explanation:

I have the detailed and self-explanatory workings. will snap and post later. Battery percent at 15%, flashlight not working.

1. The ratio of CuSO₄ to NaOH is 1 : 2

2. The limiting reactant is NaOH.

3. The mass of Cu(OH)₂ that will precipitate out is 292.5 g.

4. The percentage yield of Cu(OH)₂ is 59.7%

1. Determination of the ratio of CuSO₄ to NaOH.

CuSO₄ + 2NaOH —> Cu(OH)₂ + Na₂SO₄

From the balanced equation above, we can see that the ratio of CuSO₄ to NaOH is 1 : 2

2. Determination of the limiting reactant.

CuSO₄ + 2NaOH —> Cu(OH)₂ + Na₂SO₄

Molar mass of CuSO₄ = 63.5 + 32 + (16×4) = 159.5 g/mol

Mass of CuSO₄ from the balanced equation = 1 × 159.5 = 159.5 g

Molar mass of NaOH = 23 + 16 + 1 = 40 g/mol

Mass of NaOH from the balanced equation = 2 × 40 = 80 g

From the balanced equation above,

159.5 g of CuSO₄ reacted with 80 g of NaOH.

Therefore,

638.44 g of CuSO₄ will react with = (638.44 × 80) / 159.5 = 320.22 g of NaOH.

From the calculations made above, we can see that a higher mass (i.e 320.22 g) of NaOH than what was given (i.e 240 g) is needed to react completely with 638.44 g of CuSO₄.

Therefore, NaOH is the limiting reactant.

3. Determination of the mass of Cu(OH)₂ that will precipitate out.

CuSO₄ + 2NaOH —> Cu(OH)₂ + Na₂SO₄

Molar mass of Cu(OH)₂ = 63.5 + 2(16 + 1) = 97.5 g/mol

Mass of Cu(OH)₂ from the balanced equation = 1 × 97.5 = 97.5 g

From the balanced equation above,

80 g of NaOH reacted to produce 97.5 g of Cu(OH)₂.

Therefore,

240 g of NaOH will react to produce = (240 × 97.5) / 80 = 292.5 g of Cu(OH)₂

Thus, 292.5 g of Cu(OH)₂ precipitated out of the reaction.

4. Determination of the percentage yield.

Actual yield of Cu(OH)₂ = 174.6 gTheoretical yield of Cu(OH)₂ = 292.5 gPercentage yield of Cu(OH)₂ =?

Percentage yield = (Actual /Theoretical) × 100

Percentage yield of Cu(OH)₂ = (174.6 / 292.5) × 100

Percentage yield of Cu(OH)₂ = 59.7%

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How does specific heat capacity affect heating rate

Answers

Answer:

The specific heat capacity affect heating rate by the amount of heat needed to raise the temperature of 1 gram of a substance 1 degree Celsius (°C).

Explanation:

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Which of the following compounds has the lowest percent gold content by mass which one has the highest percent gold content by weight?

a. AuOH
b. Au(OH)3
c. AuCI3
d. AuI3

Answers

highest percentage of gold by weight can be determined by looking at the atomic mass of the other elements in the compound. the smaller the total of mass of the other elements, the heigher the percentage of Au will be in that compound

a. AuOH, OH- = 17 gram/mole
b. Au(OH)3, 3 x OH- = 3 x 17 = 51 gram/mole
c. AuCl3 = 3x35.5= 106.5 gram/mole
d. AuI3 = 3x127 = 381 gram/mole


as we can see AuOH has the least Molecular mass, hence % of Au will be the highest compared to other choices

Answer: A

Answer:D. AuI3

Explanation:

An object measures 6.2 cm×13.7 cm×26.9 cm. Which value is the length of the object?

Answers

Answer: 6.2cm

Explanation: because don’t forget it’s

Length • Width • Height

what is the chemical formula of (Sodium hydroxide ) ??​

Answers

[tex]\huge{ \color{black}{ \boxed{ \color{hotpink}{Answer}}}}[/tex]

what is the chemical formula of (Sodium hydroxide ) ??

⚘ NaOHSodium hydroxide, Sodium oxidanide

[tex] \: [/tex]

[tex]\large\tt\:⚘ \: MishiChaeYoon \: ⚘[/tex]

Answer:

The formula of Sodinm hydroxide is NaOH

Hope it helps


In your own words, summarize the contrasting arguments of Democritus and Aristotle about the composition (make up) of matter.

Answers

Answer:

Aristotle disagreed with Democritus and offered his own idea of the composition of matter. According to Aristotle, everything was composed of four elements: earth, air, fire, and water. The theory of Democritus explained things better, but Aristotle was more influential, so his ideas prevailed.

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Give the complete electronic configuration for Mn.
a. 1s22s22p63s23p64s13d6
b. 1s22s22p63s23p64s23d5
c. 1s22s22p63s23p64s24p5
d. 1s22s22p63s23p64s24d5

Answers

Answer:

B 1s2, 2s2, 2p6, 3s2, 3p6, 4s2, 3d5

Explanation:

who is th father of chemistry

Answers

Antoine Lavoisier is the father of chemistry

Identify two ways to measure mass​

Answers

Answer:

Putting it in water, and see the displacement of the water, or just measuring its weight and devide it by the gravitational pull

Scientists use a balance to obtain an object’s mass. A balance compares an object with a known mass to the object in question. One example of a balance is the triple beam balance. The standard unit of measure for mass is based on the metric system and is typically denoted as kilograms or grams.

1. Liver Balance
2. Physical balance
3. Electronic Balance

which square is more dense

Answers

Answer:

mass ,volume and density

illustrate the law of multiple proportions using the formation of SO2 and SO3 by sulphur and oxygen​

Answers

In SO2 and SO3, oxygen combines with sulphur in a fixed ratio.

The law of multiple proportion states that if two elements combine with each other to form more than one chemical compound, then the various masses of the elements which combine with a fixed mass of the other element are in simple multiple ratios.

To illustrate this law, sulphur forms two oxides, SO2 and SO3. The ratio of the oxygen that combines with a fixed mass of sulphur  in the two compounds is 2:3 in accordance to the law of multiple proportion.

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Heating a particular ether with HBr yielded a single organic product. Which of the following conclusions can be reached?
A) The reactant was a methyl ether.
B) The reactant was a symmetric ether.
C) The reactant was a cyclic ether.
D) Both B and C may be correct.

Answers

only C is correct.

A will produce either an methylbromide + alcohol

B will produce alcohol and alkyl bromide as well

C cyclic ether when reacted with HBr will only produce 1 product which has alcohol group (-OH group) on one end and Bromide group on the other end

The conclusion that can be reached is ( C ) ; The reactant was a cyclic ether

When a particular ether is heated with HBr and it yields a single organic product the only reactant that can be used to achieve this from the options listed is, Cyclic ether. because the reaction between cyclic ether and HBr will produce a single product which consists of an alcohol group ( -OH group ) and a Bromide group on each ends.

While  reactants like symmetric ether will produce alcohol and alkyl bromide

Hence the conclusion that can be reached is that the reactant used was a cyclic ether

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how can you use terms such as thermal energy, particles, motion, and flow to explain the movement of heat in our world

Answers

Answer:  Thermal energy is the kinetic energy of particles and heat is the term used to describe how this energy may flow to other systems, such as in a radiator.

Explanation:  Molecules have a level of kinetic energy that can be measured by temperature.  Higher temperatures result in more rapid and frequent collisons of particles in motion.  Heat is the term used to measure the quantity of vibration energy in a given system.  The SI unit for heat energy is the Joule.  The water molecules in a pot of boiling water have a kinetic energy sufficient for them to break away from other water molecules and become gaseous (boiling point).  The heat energy in 1 liter of water at 95C can be calculated in terms of Joules/liter, based on the specific heat of water (e.g., in units of J/gC).  This heat can flow to other systems.  A home boiler heater unit heats water tha can be pumped around the house ehre it will flow from radiators into thje surrounding air.

Heat is the word used to explain how this energy may transfer to other systems, such as in a radiator, and thermal energy is the kinetic energy of particles.

What is thermal energy?

By virtue of its temperature, a system in a condition of thermodynamic equilibrium has thermal energy or internal energy. Unlike the energy of systems that are not in a state of thermodynamic equilibrium, thermal energy cannot be transformed into meaningful work as quickly.

For instance, the same fluid or solid in a thermodynamic equilibrium state with the same energy (as thermal energy) cannot do work unless it is combined with another substance at a different temperature, as in a heat engine. However, the same fluid or solid in a moving state with the same energy (as mechanical energy) can be converted to work in some mechanical device, such as a windmill or a waterwheel.

Therefore, heat is the word used to explain how this energy may transfer to other systems, such as in a radiator, and thermal energy is the kinetic energy of particles.

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The one factor that a scientist changes in an experiment is called the
O
A responding variable.
O
B. hypothesis.
C. controlled variable.
D. manipulated variable.

Answers

Answer:

It might be responding variable.

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